Publications

• Liu Y, Liu YS, Niu XR, Chen A, Li YZ, Yu Y, Mo BR, Liu ZH, Xu T, Cheng J, Wu ZG, Wei WS. (2025) Massively parallel interrogation of human functional variants modulating cancer immunosurveillance. Signal Transduction and Targeted Therapy 10, 88.
• Qin SJ, Zhang XY, Zhang Y, Miao DY, Wei WS, Bai Y. (2025) Multi-dimensional bio mass cytometry: simultaneous analysis of cytoplasmic proteins and metabolites on single cells. Chemical Science DOI: 10.1039/d4sc05055j.
• Zhang XX, Zhang X, Ren JW, Li JY, Wei XX, Yu Y, Yi ZY, Wei WS. (2025) Precise modelling of mitochondrial diseases using optimized mitoBEs. Nature https://doi.org/10.1038/s41586-024-08469-8.
• He X, Liu YS, Gao X, Tang FY, Tian YX, Gong SY, Shen J, Wang AM, Sun LQ, Wei WS, Weng L. (2024) N-terminal acetylation of transcription factor LIP induces immune therapy resistance via suppression of PD-L1 expression in non-small cell lung cancer. Journal for ImmunoTherapy of Cancer 12, e009905.
• Pan Q, Zhang ZX, Xiong YF, Bao Y, Chen TX, Xu P, Liu ZH, Ma HZ, Yu Y, Zhou Z, Wei WS. (2024) Mapping functional elements of the DNA damage response through base editor screens. Cell Reports 43, 115047.
• Bao Y, Wei WS. (2024) Protocol for high-throughput screening of functional lysine residues in cell fitness. STAR Protocols 5 (4) 103418.
• Li YZ, Xu T, Ma HZ, Yue D, Lamao QZ, Liu Y, Zhou Z, Wei WS. (2024) Functional profiling of serine, threonine and tyrosine sites. Nature Chemical Biology https://doi.org/10.1038/s41589-024-01731-0
• Yi ZY, Zhang XX, Wei XX, Li JY, Ren JW, Zhang X, Zhang YK, Tang HX, Chang XW, Yu Y, Wei WS. (2024) Programmable DNA pyrimidine base editing via engineered uracil-DNA glycosylase. Nature Communications 15, 6397.
• Zhang XX, Yi ZY, Tang W, Wei WS. (2024) Streamlined process for effective and strand-selective mitochondrial base editing using mitoBEs. Biophysics Reports 10(4): 191-200.
• Wu ZG, Lamao QZ, Gu MC, Jin XX, Liu Y, Tian F, Yu Y, Yuan PF, Gao SX, Fulford TS, Uldrich AP, Wong CCL and Wei WS. (2024) Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing. Cellular Molecular Immunology 21, 362–373.
• Ma KY, Wang X, Wu LJ, Yu LL, YeJH, Li XL, Geng LL, Shi ZY, Yang HH, Zhang XJ, Zhang YJ, Wu SC, Yuan PF, Zhang YC, Dong F, Hao S, Hu LP, Wei WS, Fang RG, and Cheng T. (2023) CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment. iScience 26(12) 108561.
• Bao Y, Pan Q, Xu P, Liu ZH, Zhang ZX, Liu YS, Xu YY, Yu Y, Zhou Z, Wei WS. (2023) Unbiased interrogation of functional lysine residues in human proteome. Molecular Cell 83, 1-19.
• Yi ZY, Zhao YX, Yi ZX, Zhang YJ, Tang GB, Zhang XX, Tang HX, Zhang W, Zhao Y, Xu HY, Nie YY, Sun XQ, Xing LJ, Dai L, Yuan PY and Wei WS. (2023) Utilizing AAV-mediated LEAPER 2.0 for programmable RNA editing in non-human primates and nonsense mutation correction in humanized Hurler syndrome mice. Genome Biology 24, 243.
• Yi ZY, Zhang XX, Tang W, Yu Y, Wei XX, Zhang X and Wei WS. (2023) Strand-selective base editing of human mitochondrial DNA using mitoBEs. Nature Biotechnology 42, 498–509.
• Zhang XX and Wei WS. (2023) CRISPR-free, strand-selective mitochondrial DNA base editing using a nickase. Nature Biotechnology 42, 392–393.
• Mi L, Shi M, Li YX, Xie G,Rao XC, Wu DM, Cheng AM, Niu MX, Xu FL, Yu Y, Gao N, Wei WS, Wang XH and Wang YM. (2023) DddA homolog search and engineering expand sequence compatibility of mitochondrial base editing. Nature Communications 14, 874.
• Chen XP, Qu X, Liu CC, Zhang Y, Zhang GG, Han P, Duan YL, Li Q, Wang L, Ruan WJ, Wang PY, Wei WS, Gao GF, Zhao X and Xie ZD. (2022) Human FcRn Is a Two-in-One Attachment-Uncoating Receptor for Echovirus 18. mBio e01166-22.
• Liu Y, Ding B, Zheng LN, Xu P, Liu ZH, Chen Z, Wu PY, Zhao Y, Pan Q, Guo Y, Wang W and Wei WS. (2022) Regulatory elements can be essential for maintaining broad chromatin organization and cell viability. Nucleic Acids Research 50(8): 4340-4354.
• Qu L, Yi ZY, Shen Y, Lin LR, Chen F, Xu YY, Wu ZG, Tang HX, Zhang XX, Tian F, Wang CH, Xiao X, Dong XJ, Guo L, Lu SY, Yang CY, Tang C, Yang Y, Yu WH, Wang JB, Zhou YN, Huang Q, Yisimayi A, Liu S, Huang WJ, Cao YL, Wang YC, Zhou Z, Peng XZ, Wang JW, Xie XS and Wei WS. (2022) Circular RNA Vaccines against SARS-CoV-2 and Emerging Variants. Cell 185(10): 1728-1744.
• Wei WS and Gao CX. (2022) Gene editing: from technologies to applications in research and beyond. Science China Life Sciences 65, 657-659.
• Li GL, Li XY, Zhuang SK, Wang LR, Zhu YF, Chen YC, Sun W, Wu ZG, Zhou Z, Chen J, Huang XX, Wang J, Li DL, Li W, Wang HY and Wei WS. (2022) Gene editing and its applications in biomedicine. Science China Life Sciences 65, 660-700.
• Yi ZY, Qu L, Tang HX, Liu ZH, Liu Y, Tian F, Wang CH, Zhang XX, Feng ZQ, Yu Y, Yuan PF, Yi ZX, Zhao YX and Wei WS. (2022) Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo. Nature Biotechnology 40, 946-955.
• Ding B, Liu Y, Liu ZH, Zheng LN, Xu P, Chen Z, Wu PY, Zhao Y, Pan Q, Guo Y, Wei WS and Wang W. (2021) Noncoding loci without epigenomic signals can be essential for maintaining global chromatin organization and cell viability. Science Advances 7(45): eabi6020.
• Zhou Z, Zhang XY, Lei XB, Xiao X, Jiao T, Ma RY, Dong XJ, Jiang Q, Wang WJ, Shi YJ, Zheng T, Rao J, Xiang ZC, Ren LL, Deng T, Jiang ZF, Dou ZX, Wei WS and Wang JW. (2021) Sensing of cytoplasmic chromatin by cGAS activates innate immune response in SARS-CoV-2 infection. Signal Transduction and Targeted Therapy 6, 382.
• Zhu SY, Liu Y, Zhou Z, Zhang ZY, Xiao X, Liu ZH, Chen A,Dong XJ, Tian F, Chen SH, Xu YY, Wang CH, Li QH, Niu XR, Pan Q, Du S, Xiao JY, Wang JW and Wei WS. (2021) Genome-wide CRISPR activation screen identifies candidate receptors for SARS-CoV-2 entry. Science China Life Sciences https://doi.org/10.1007/s11427-021-1990-5.
• Guo SJ, Chen YO, Liu JZ, Zhang XY, Liu ZH, Zhou Z and Wei WS. (2021) Low-density lipoprotein receptor-related protein 1 is a CROPs-associated receptor for Clostridioides difficile toxin B. Science China Life Sciences 65, 107–118.
• Xu P, Liu ZH, Liu Y, Ma HZ, Xu YY, Bao Y, Zhu SY, Cao ZZ, Wu ZG, Zhou Z and Wei WS. (2021) Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs. Nature Biotechnology 39, 1403–1413.
• Liang YS, Zhang GG, Li QH, Han L, Hu XY, Guo Y, Tao WY, Zhao XM, Guo MZ, Gan TY, Tong YM, Xu YF, Zhou Z, Ding Q, Wei WS and Zhong J. (2021) TRIM26 is a critical host factor for HCV replication and contributes to host tropism. Science Advances 7, eabd9732.
• Lei XB, Dong XJ, Ma RY, Wang WJ, Xiao X, Tian ZQ, Wang CH, Wang Y, Li L, Ren LL, Guo F, Zhao ZD, Zhou Z, Xiang ZC and Wang JW. (2020). Activation and evasion of type I interferon responses by SARS-CoV-2. Nature Communications 11(1), 3810.
• Zhou Z, Ren LL, Zhang L, Zhong JX, Xiao Y, Jia ZL, Guo L, Yang J, Wang C, Jiang S, Yang DH, Zhang GL, Li HR, Chen FH, Xu Y, Chen MW, Gao ZC, Yang J, Dong J, Liu B, Zhang XN, Wang WD, He KL, Jin Q, Li MK and Wang JW. (2020). Heightened Innate Immune Responses in the Respiratory Tract of COVID-19 Patients. Cell Host Microbe 27, 883-890.
• Liu Y, Liu ZH, Cao ZZ and Wei WS. (2020). Reply to: Fitness effects of CRISPR/Cas9-targeting of long noncoding RNA genes. Nature Biotechnology 38, 577-578.
• Liu LL, Zhang Y, Liu MH, Wei WS, Yi CQ and Peng JY. (2019). Structural Insights into the Specific Recognition of 5-methylcytosine and 5-hydroxymethylcytosine by TAL Effectors. Journal of Molecular Biology 432(4), 1035-1047.
• Zhang XY, Yue D, Wang YN, Zhou YX, Liu Y, Qiu YT, Tian F, Yu Y, Zhou Z and Wei WS. (2019). PASTMUS: mapping functional elements at single amino acid resolution in human cells. Genome Biology 20, 279.
• Qu L, Yi ZY, Zhu SY, Wang CH, Cao ZZ, Zhou Z, Yuan PF, Yu Y, Tian F, Liu ZH, Bao Y, Zhao YX and Wei WS. (2019). Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs. Nature Biotechnology 37, 1059-1069.
• Zhao X, Zhang GG, Liu S, Chen XP, Peng RC, Dai LP, Qu X, Li SH, Song H, Gao ZR, Yuan PF, Liu ZH, Li CY, Shang ZF, Li Y, Zhang MF, Qi JX, Wang H, Du N, Wu Y, Bi YH, Gao S, Shi Y, Yan JH, Zhang Y, Xie ZD, Wei WS and Gao GF. (2019). Human Neonatal Fc Receptor Is the Cellular Uncoating Receptor for Enterovirus B. Cell 177, 1553-1565.
• Zhang GG, Zhou Z and Wei WS. (2019). In vivo ways to unveil off-targets. Cell Research 29, 339-340.
• Lander ES, Baylis F, Zhang F, Charpentier E, Berg P, Bourgain C, Friedrich B, Joung JK, Li JS, Liu D, Naldini L, Nie JB, Qiu RZ, Schoene-Seifert B, Shao F, Terry S, Wei WS and Winnacker EL. (2019). Adopt a moratorium on heritable genome editing. Nature 567, 165-168.
• Zhu SY, Cao ZZ, Liu ZH, He Y, Wang YN, Yuan PF, Li W, Tian F, Bao Y and Wei WS. (2019). Guide RNAs with embedded barcodes boost CRISPR-pooled screens. Genome Biology 20, 20.
• Wang HY, Li JS, Li W, Gao CX and Wei WS. (2018). CRISPR twins: a condemnation from Chinese academic societies. Nature 564, 345.
• Zhou Z and Wei WS. (2018). Interrogating the noncoding genome in a high-throughput fashion. National Science Review 6, 397-399.
• Chen YO, Bao Y, Ma HZ, Yi ZY, Zhou Z and Wei WS. (2018). Gene editing technology and its research progress in China. Hereditas 40, 900-915.
• Liu Y, Cao ZZ, Wang YN, Guo Y, Xu P, Yuan PF, Liu ZH, He Y and Wei WS. (2018). Genome-wide screening for functional long noncoding RNAs in human cells by Cas9 targeting of splice sites. Nature Biotechnology 36, 1203-1210.
• Zhou Z and Wei WS. (2018). PrePAIRing Cas9s for screening success. Nature Biotechnology 36, 147-148.
• Zhang HM, Zhou YX, Wang YN, Zhao YG, Qiu YT, Zhang XY, Yue D, Zhou Z and Wei WS. (2018). A surrogate reporter system for multiplexable evaluation of CRISPR/Cas9 in targeted mutagenesis. Scientific Reports 8, 1042.
• Fan HH, Qiao LH, Kang KD, Fan JF, Wei WS and Luo GX. (2017). Attachment and Postattachment Receptors Important for Hepatitis C Virus Infection and Cell-to-Cell Transmission. Journal of Virology 91, 1-20.
• He RN, Peng JY, Yuan PF, Yang JJ, Wu XJ, Wang YN and Wei WS. (2017). Glucosyltransferase Activity of Clostridium difcile Toxin B Triggers Autophagy-mediated Cell Growth Arrest. Scientific Reports 7, 10532.
• Hu H, Zhang HM, Wang S, Ding M, An H, Hou YP, Yang XJ, Wei WS, Sun YJ and Tang C. (2017). Live visualization of genomic loci with BiFC-TALE. Scientific Reports 7, 40192.
• Zhang Y, Liu LL, Guo SJ, Song JH, Zhu CX, Yue ZW, Wei WS and Yi CQ. (2017). Deciphering TAL effectors for 5-methylcytosine and 5-hydroxymethylcytosine recognition. Nature Communications 8, 901.
• Zhou YX, Wang P, Tian F, Gao G, Huang L, Wei WS and Xie XS. (2017). Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging. Cell Research 27, 298-301.
• Zhu SY, Zhou YX and Wei WS. (2017). Genome-Wide CRISPR/Cas9 Screening for High-Throughput Functional Genomics in Human Cells. Methods in Molecular Biology 1656, 175-181.
• Burgess S, Cheng LZ, Gu F, Huang JJ, Huang ZW, Lin S, Li JS, Li W, Qin W, Sun YJ, Zhou SY, Wei WS, Wu Q, Wang HY, Wang XQ, Xiong JW, Xi JZ, Yang H, Zhou B and Zhang B. (2016). Questions about NgAgo. Protein Cell 7, 913-915.
• Shao SP, Zhang WW, Hu H, Xue BX, Qin JS, Sun CY, Sun Y, Wei WS and Sun YJ. (2016). Long-term dual-color tracking of genomic loci by modified sgRNAs of the CRISPR/Cas9 system. Nucleic Acids Research 44, e86.
• Yang JJ, Guo SJ, Yuan PF and Wei WS. (2016) . Assembly of Customized TAL Effectors Through Advanced ULtiMATE System. Methods in Molecular Biology 1338, 49-60.
• Zhou YX and Wei WS. (2016). Mapping regulatory elements. Nature Biotechnology 34, 151-152.
• Zhou YX, Zhang HM and Wei WS. (2016). Simultaneous generation of multi-gene knockouts in human cells. FEBS Letters 590, 4343-435.
• Zhu SY and Wei WS. (2016). Making better CRISPR libraries. eLife 5, e21863.
• Zhu SY, Li W, Liu JZ, Chen CH, Liao Q, Xu P, Xu H, Xiao TF, Cao ZZ, Peng JY, Yuan PF, Brown M, Liu XS and Wei WS. (2016). Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Nature Biotechnology 34, 1279-1286.
• He RN, Peng JY, Yuan PF, Xu F and Wei WS. (2015). Divergent roles of BECN1 in LC3 lipidation and autophagosomal function. Autophagy 11, 740-747.
• Peng JY, Zhou YX, Zhu SY and Wei WS. (2015). High-throughput screens in mammalian cells using the CRISPR-Cas9 system. FEBS Journal 282, 2089-2096.
• Ren QP, Li C, Yuan PF, Cai CZ, Zhang LQ, Luo GG and Wei WS. (2015). A Dual-Reporter System for Real-Time Monitoring and High-throughput CRISPR/Cas9 Library Screening of the Hepatitis C Virus. Scientific Reports 5, 8865.
• Shen J, Cai CZ, Yu ZL, Pang YH, Zhou Y, Qian LL, Wei WS and Huang YY. (2015). A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium. Scientific Reports 5, 8651.
• Yuan PF, Zhang HM, Cai CZ, Zhu SY, Zhou YX, Yang XZ, He RN, Li C, Guo SJ, Li S, Huang TX, Perez-Cordon G, Feng HP and Wei WS. (2015). Chondroitin sulfate proteoglycan 4 functions as the cellular receptor for Clostridium difficile toxin B. Cell Research 25, 157-168.
• Qian LL, Cai CZ, Yuan PF, Jeong SY, Yang XZ, Dealmeida V, Ernst J, Costa M, Cohen SN and Wei WS. (2014). Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake. Science China-Life Sciences 57, 469-481.
• Yang JJ, Zhang Y, Yuan PF, Zhou YX, Cai CZ, Ren QP, Wen DQ, Zhu C, Qi H and Wei WS. (2014). Complete decoding of TAL effectors for DNA recognition. Cell Research 24, 628-631.
• Zhou YX, Zhu SY, Cai CZ, Yuan PF, Li CM, Huang YY and Wei WS. (2014). High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature 509, 487-491.
• Yang JJ, Yuan PF, Wen DQ, Sheng Y, Zhu SY, Yu YZ, Gao X and Wei WS. (2013). ULtiMATE System for Rapid Assembly of CustomizedTAL Effectors. PLoS One 8, e75649.